Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples.

Modulated Raman Spectroscopy for Enhanced Cancer Diagnosis at the Cellular Level

Raman spectroscopy is emerging as a promising and novel biophotonics tool for non-invasive, real-time diagnosis of tissue and cell abnormalities. However, the presence of a strong fluorescence background is a key issue that can detract from the use of Raman spectroscopy in routine clinical care. The review summarizes the state-of-the-art methods to remove the fluorescence background and explore...

Biosensing Based on Surface-Enhanced Raman Spectroscopy by Using Metal Nanoparticles

Surface-enhanced Raman spectroscopy (SERS) is a promising tool in the analytical science because it provides good selectivity and sensitivity without the labeling process required by fluorescence detection. This technique consists of locating the target analyte on nanometer range of roughed Au-nanoparticles. The presence of the metal nanoparticles provides a tremendous enhancement to the result...

Evaluation of Sensitivity and Specificity of Urine Survivin As A New Molecular Marker in Diagnosis of Bladder Tumors

Bladder cancer is one of the most common forms of cancers in the world. The current gold standards for itsdiagnosis are cystoscopy and urine cytology. Cystoscopy, a naked eye assessment of the bladder, is invasive, uncomfortable and costly with a great deal of personal variability in its results; while urine cytology has high specificity but low sensitivity, particularly for low-grade les...

Optimisation of Wavelength Modulated Raman Spectroscopy: Towards High Throughput Cell Screening

In the field of biomedicine, Raman spectroscopy is a powerful technique to discriminate between normal and cancerous cells. However the strong background signal from the sample and the instrumentation affects the efficiency of this discrimination technique. Wavelength Modulated Raman spectroscopy (WMRS) may suppress the background from the Raman spectra. In this study we demonstrate a systemati...

Discrimination of bladder cancer cells from normal urothelial cells with high specificity and sensitivity: Combined application of AFM and modulated Raman spectroscopy